Proteomics And Metabolomics Unit

Mission

To provide intellectual and technical expertise, and instrumentation (Mass Spectrometry based platform), for multiplex analysis of proteins and metabolites towards crop improvement. Importantly, to accomplish this mission, the Unit interacts closely with other research disciplines to promote and facilitate translational research and experienced research groups in data processing and data mining.
 

Strategic Research Areas

  1. Development of methods for proteomics and metabolomics profiling from important traits/planting materials of the future
  2. Detection of proteins and metabolites towards biomarkers for superior yield, oil quality, specialty oils and oil palm disease
  3. Geographical traceability and authenticity studies of palm oil to support industry QC, especially with respect to sustainability, and
  4. Metabolic reconstruction and mapping of proteomics and metabolomics data.

Proteomics GroupMetabolomics Group

Proteomics Group

Metabolomics, as promising approaches to translational research will be the main focus areas under this group. Major activities of the group include:

  1. Profiling of proteins and metabolites from the planting materials of the future (e.g. specialty oils) and Ganoderma disease
  2. Development of innovative key technologies for the plant oil market to support oil palm geographical traceability and sustainability
  3. Development and refinement of metabolomics data quality, statistical analysis and Oil Palm Metabolome Database (OPDMB)

Group Leader

Members

MS. NOOR IDAYU MHD TAHIR
Research Officer

MDM. NURAZAH ZAIN

Research Officer

MDM NURUL LIYANA ROZALI
Research Officer

Metabolomics Group

Proteomics and functional interpretation using advanced IT-learning tools will be the main focus areas of the group;

  1. Development of tissue-specific expression atlas and proteome map by linking datasets from protein profiling of palms with desired traits such as superior yield, oil quality and Ganoderma tolerance to relevant gene and transcript datasets;
  2. Development of omics data analysis pipeline using various methods including multivariate analysis, machine learning solutions, mathematical modelling and network analysis
  3. Identification of critical metabolite networks and pathways of important roles in the metabolic regulation of specific traits.

Group Leader

Members

MDM. SYAHANIM SHAHWAN


Research Officer

MDM. HASLIZA HASSAN

Research Officer

MDM. NUR ‘AIN MOHD ISHAK


Research Officer

MDM. SHAHIRAH BALQIS DZULKAFLI

Research Officer

Protein Identification with MALDI-TOF/TOF Mass Spectrometry Gas Chromatography Time-of-Flight Mass Spectrometer (GC/Q-TOF MS)

We offer to identify your protein(s) by peptide mass spectrometric analysis using a Matrix-assisted Laser Desorption/Ionization-Time-of-Flight mass spectrometer (Bruker Ultraflex III) followed by a database search with Bruker ProteinScape data management software.

Typically, after protein separations on polyacrylamide gel (1D or 2D), we will perform tryptic digest on the proteins for peptide analysis. The generated peak list is then searched against public protein sequence databases such as NCBI or SWISSPROT. Our services are paid services but the offered technologies and expertise are also attainable through collaborations.

Our service offering includes, but not limited to;

  1. Electrophoresis gel-based protein separation
  2. Enzymatic digestion with trypsin
  3. Peptide clean-up
  4. Standard protein identification
  5. Customized services (training, hands-on, project design and consultation)

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Service form for MPOB staff

Pricing

1D gel up to 10 wells – Coomassie stained
Sample preparation, run gel, staining with Coomassie, image capture with a scanner


In-gel Digestion
To include reduction and alkylation; peptide clean-up


Insolution Digestion
To include reduction and alkylation, peptide clean-up


Basic protein identification
MS and MS/MS data acquisition, database search and report


Customized services
Enquiry

Workflow

Inquiry/DiscussionGet a qoute

 



Complete the
service form
Download form for public
Download form for MPOB staff

 

 

Service begins
Report

Contact person
If you need further information, please talk to us.

Dr. Abrizah Othman
Group Leader
Proteomics Group

Dr. Benjamin Lau Yii Chung
Group Leader
Metabolomics Group

FAQs

The GC/Q-TOF MS system comprising of Gerstel 1.4.6.5 Autosampler, a 7890B Agilent Gas Chromatography and 7200B Agilent Time-of-Flight MS.

Transfer of services

MPOB TS No. 167, July 2017
Identification of Leaf Metabolome Content for Development of New Varieties


APPLY NOW!

MPOB staff application form

Pricing

NoServicesCharges per injection
(excluding 6% GST)
AcademicIndustry
1Metabolite analysis with derivatization N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA)RM 200.00RM 250.00
2Dynamic headspace (DHS) /headspace for volatile organic compounds (VOCs) analysisRM 150.00RM 200.00

Contact person
If you need further information, please contact our officer

Dr. Abrizah Othman

Group Leader
Translational OMICS Group

MDM NURUL LIYANA ROZALI

Research officer
Translational OMICS Group